Antigens in Solution - Hooke Laboratories
Suitable for in vivo and in vitro stimulation of immune cells.
Antigens in PBS are typically used for in vivo challenge, after immunization of animals with the same antigen in adjuvant.
Antigens in tissue culture (TC) media are most often used in vitro, for restimulation of spleen or lymph node cells isolated from immunized animals.
Antigens in PBS
| Cat # | Product | Description | Size |
| DS-0142 | Ovalbumin (OVA) in PBS | Protein in PBS | 2 mg in 1 mL |
| DS-0162 | Methylated bovine serum albumin (mBSA) in PBS | Protein in PBS | 5 mg in 1 mL |
DS-0142 : Ovalbumin (OVA) in PBS
For immunization of laboratory animals
Chicken ovalbumin (OVA) protein in PBS, ready to use
Sterile filtered, supplied in vials
Detailed contents
- Chicken ovalbumin (OVA) protein
- 2 mg in 1 mL of phosphate buffered saline (PBS)
- Sterile filtered
- Supplied in vials, ready to use
Storage & stability
- Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
- Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0162 : Methylated bovine serum albumin (mBSA) in PBS
For induction of DTH
In PBS, ready to use
Supplied in vials
For induction of DTH
Challenge with mBSA consistently induces DTH recall response in mBSA immunized mice
Protocols
Induction of DTH in C57BL/6 Mice
Detailed contents
Methylated bovine serum albumin (mBSA)
5 mg in 1 mL of phosphate buffered saline (PBS)
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
Antigens in TC media
| Cat # | Product | Description | Size |
| DS-0111 | MOG35-55 in TC media | Peptide in tissue culture media | 2 mg in 1 mL |
| DS-0121 | [Ser140]-PLP139-151 in TC media1 | Peptide in tissue culture media | 2 mg in 1 mL |
| DS-0131 | MBP1-17 in TC media | Peptide in tissue culture media | 2 mg in 1 mL |
| DS-0141 | OVA323-339 in TC media | Peptide in tissue culture media | 2 mg in 1 mL |
| DS-0151 | gpMBP69-88 in TC media | Peptide in tissue culture media | 2 mg in 1 mL |
| DS-0161 | PLP139-151 (native) in TC media | Peptide in tissue culture media | 2 mg in 1 mL |
DS-0111 : MOG35-55 in TC media
For in vitro restimulation of MOG35-55-specific T cells
Tissue culture tested
In tissue culture media, ready to use : Supplied in vials
For analysis of T cell functions in vitro :Consistently induces in vitro recall responses of T cells isolated from MOG35-55/CFA-immunized mice
For adoptive transfer EAE in C57BL/6 mice : Induces potent encephalitogenic T cells in vitro when used with IL-12 and anti-interferon-gamma (anti-IFN-γ) antibodies
MOG35-55 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells from immunized C57B/6 mice, IL-12 and anti IFNγ antibodies are added to the culture.
Protocols
Adoptive Transfer EAE in C57BL/6 Mice
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
Myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) rat, mouse
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence MEVGWYRSPFSRVVHLYRNGK
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0121 : [Ser140]-PLP139-151 in TC media
For in vitro restimulation of [Ser140]-PLP139-151-specific T cells
Tissue culture tested
In tissue culture media, ready to use : Supplied in vials
For analysis of T cell functions in vitro : Consistently induces in vitro recall responses of T cells isolated from [Ser140]-PLP139-151/CFA-immunized mice
For adoptive transfer EAE in SJL mice : Induces potent encephalitogenic T cells in vitro
[Ser140]-PLP139-151 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes and lymph node cells from immunized mice for 72 hours in the presence of 20 µg/mL of antigen.
Protocols
Adoptive Transfer EAE in SJL Mice
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
[Ser140]-PLP139-151 (serine substituted myelin proteolipid protein 139-151)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence HSLGKWLGHPDKF
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0131 : MBP1-17 in TC media
For in vitro restimulation of MBP1-17-specific T cells
Tissue culture tested
In tissue culture media, ready to use : Supplied in vials
For analysis of T cell functions in vitro : Consistently induces in vitro recall responses of T cells isolated from MBP1-17/CFA-immunized mice : For adoptive transfer EAE in B10.PL, PL/J, or anti-MBP TCR transgenic mice
Induces potent encephalitogenic T cells in vitro
MBP1-17 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells, IL-23 or IL-12 and anti-IFNγ antibodies may be added to the culture.
Protocol
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
Myelin basic protein 1-17 (MBP1-17)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Mouse sequence ASQKRPSQRSKYLATAS
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0141 : OVA323-339 in TC media
For in vitro restimulation of OVA323-339-specific T cells
Tissue culture tested
In tissue culture media, ready to use : Supplied in vials
For analysis of T cell functions in vitro : Consistently induces in vitro recall responses of T cells isolated from OVA323-339/CFA-immunized mice
Consistently induces in vitro stimulation of DO11.10 (H-2d) and OT-II (H-2b) cells
Protocols
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
Chicken ovalbumin peptide 323-339 (OVA323-339)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence ISQAVHAAHAEINEAGR
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0151 : gpMBP69-88 in TC media
For in vitro restimulation of gpMBP69-88-specific T cells
Tissue culture tested
In tissue culture media, ready to use :Supplied in vials
For analysis of T cell functions in vitro
Detailed contents
Guinea pig myelin basic protein peptide 69-88 (gpMBP69-88)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence YGSLPQKSQRSQDENPVVHF
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
DS-0161 : PLP139-151 (native) in TC media
For in vitro restimulation of PLP139-151-specific T cells
Tissue culture tested
In tissue culture media, ready to use : Supplied in vials
For analysis of T cell functions in vitro : Consistently induces in vitro recall responses of T cells isolated from PLP139-151/CFA-immunized mice
For adoptive transfer EAE in SJL mice: Induces potent encephalitogenic T cells in vitro
PLP139-151 in TC Media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes and lymph node cells from immunized mice for 72 hours in the presence of 20 µg/mL of antigen.
Protocols
Adoptive Transfer EAE in SJL Mice
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
PLP139-151 (myelin proteolipid protein 139-151)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence HCLGKWLGHPDKF
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.
Contact & Ask
MDKLAB
www.mdklab.co.kr
TEL : 02 553 0923